ABSTRACT
Objective: To establish an HPLC-DAD method for the determination of rutin and hyperoside in the leaves of "WudangⅡ Flos Lonicerae". Methods: A Fortis Xi Phenyl column (250 mm×4. 6 mm, 5 μm) was adopted, the mobile phase was acetoni-trile-0. 5% glacial acetic acid aqueous solution with gradient elution at a flow rate of 0. 9 ml·min-1, the detection wavelength was 354 nm, and the column temperature was 30℃. Results: The linear range of rutin (r=0. 999 5) and hyperoside (r=0. 999 5) was 9. 00-90. 00μg·ml-1and 16. 35-163. 50 μg·ml-1, and the average recovery was 99. 70% ( RSD =1. 96% ) and 99. 30% ( RSD =1. 95% ), respectively. Conclusion: The method is simple, accurate, reproducible and specific, which can be used for the determina-tion of rutin and hyperoside in the leaves of "Wudang Ⅱ Flos Lonicerae".